Use of a simple enzymatic assay for cholesterol analysis in human bile.

Use of a simple enzymatic assay for cholesterol analysis in human bile.

An enzymatic technique for cholesterol analysis in serum was applied to human bile. The analytical yield was very satisfactory in experiments in which known amounts of cholesterol were added to untreated, as well as Millipore-filtered, samples of human bile. The analytical results of the enzymatic test agreed closely with those of a method utilizing the Liebermann-Burchard reaction. The enzymat...

Simple enzymatic assay for determining cholesterol concentrations in bile.

We use bilirubin oxidase (EC 1.3.3.5) to remove interference by bilirubin in the assay of cholesterol concentration in bile by standard enzymatic methods. Samples are treated for 10 min with nonlimiting amounts of bilirubin oxidase to form biliverdin from bilirubin before the reagent for cholesterol is added. The relatively small interference by biliverdin is easily eliminated by use of sample ...

a time-series analysis of the demand for life insurance in iran

با توجه به تجزیه و تحلیل داده ها ما دریافتیم که سطح درامد و تعداد نمایندگیها باتقاضای بیمه عمر رابطه مستقیم دارند و نرخ بهره و بار تکفل با تقاضای بیمه عمر رابطه عکس دارند

A simple method for the determination of serum cholesterol

Enzymatic assay for cholesterol ester hydrolase activity.

A rapid and accurate method is described for the assay of cholesterol ester hydrolase (CEH) activity. Aliquots of the enzyme-substrate incubation mixture are extracted into isopropanol. The free cholesterol concentration in each extract is determined enzymatically using a single aqueous reagent containing cholesterol oxidase and peroxidase. The free cholesterol remaining after the cholesterol e...